What is a notable feature of Sanger sequencing compared to Next Generation sequencing?

Sanger sequencing is primarily characterized by single fragment analysis, which is one of its most notable features. This method focuses on sequencing one DNA fragment at a time, typically up to about 700-1000 base pairs in length. This approach allows for high accuracy when obtaining the sequence of shorter DNA segments, making Sanger sequencing particularly effective for applications that require precise results from individual fragments, such as confirming variants detected by other methods or for small-scale projects.

In contrast, Next Generation Sequencing (NGS) operates on a different principle, allowing for the simultaneous analysis of thousands to millions of fragments in parallel. This high-throughput capability enables researchers to sequence entire genomes or large regions very quickly and efficiently, which is not feasible with Sanger sequencing. Understanding the distinction between these two sequencing technologies is critical for genetic counselors and researchers when selecting appropriate methods for different types of genetic analysis.